TL;DR
The preparation and analysis of standard, miRNA-enriched libraries that contain 5'-monophosphorylated RNAs and 3'-hydroxyl ends are described, as well as a custom protocol for the analysis of sRNAs containing 5'-cap modifications.
This chapter explores the biomedical significance of ceRNAs, highlighting their role in modulating gene expression and their potential implications in disease, and summarizes available tools, resources, and practical examples for studying lncRNA-miRNA interactions to aid researchers in navigating this sophisticated reg...
By integrating rRNA and snoRNA sequencing approaches, this work has built a comprehensive profile of rRNA modification dynamics during early embryonic cell fate decisions, highlighting potential regulatory mechanisms for ribosome heterogeneity during development.
Two complementary methods for tsRNA validation are presented, small RNA northern blotting, which allows direct visualization of RNA size and abundance, and dumbbell PCR, which enables isoform-specific quantification with single-nucleotide resolution, which provide robust tools to validate tsRNA expression and facilitat...
Current strategies for detecting and mapping RNA modifications in the small RNome include demodification-based sequencing approaches for heavily modified RNAs, targeted chemical and enzymatic methods for site-specific mapping, and emerging direct RNA sequencing technologies that allow analysis of native molecules witho...
It is proposed that the upregulated lncRNA ENSG00000265613 may enhance malignancy by stabilizing the RNA target ENSG00000582008 in luminal A breast cancer, particularly given its established role in oncogenesis.
How circRNAs regulate homeostasis in normal tissues, how dysregulated expression of circRNAs impacts various cancers and emerging therapeutic technologies that build upon their unique features are reviewed.
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