Heterologous Expression of the Bean Chitinase Gene (ch5B) in Lettuce (Lactuca sativa L.) Confers Enhanced Tolerance to Rhizoctonia solani and Demonstrates Agronomic Substantial Equivalence in Field Trials
Fungal pathogens cause severe yield losses in lettuce production worldwide. We developed transgenic lettuce lines overexpressing the ch5B chitinase gene from Phaseolus vulgaris (bean) under the regulation of the rbcS1 (ribulose-1,5-bisphosphate carboxylase small subunit) promoter. Molecular characterization confirmed stable transgene integration and expression across the selected lines. In vitro assays demonstrated that transgenic leaf extracts significantly inhibited Rhizoctonia solani mycelial growth. Furthermore, inoculation assays in both seedlings and adult plants in the greenhouse showed a significant reduction in lesion areas compared to non-transgenic (NT) controls. Finally, two lines were evaluated in field trials, both showing agronomic substantial equivalence to the NT, satisfying international biosafety frameworks for commercial development. Among them, the ch5B 3-4 line appears to be the most promising candidate for further studies, as it showed slightly higher fresh weight compared to the NT under field conditions.:
The results showed that HS09 activates key defense-related signaling pathways, particularly those mediated by jasmonic acid (JAMyb and MYC2) and ethylene (EIN3, EILs, and ERF transcription factors), and induction of the phenylpropanoid biosynthesis pathway, promoting the production of antimicrobial secondary metabolite...
Zafiro Barraza Román, Héctor Alejandro Rodríguez Cabal, Zulma Isabel Monsalve F et al.· Journal of plant diseases an...· 0 citations
The AhDef1 gene functionally validated in this study emerges as a promising candidate for engineering fungal disease resistance and aflatoxin mitigation in peanut and potentially other susceptible crops.
Nirmala Kumari Gupta, Jaykumar Patel, Gohil Mrunaliniba Yuvrajsinh et al.· Current Issues in Molecular...· 0 citations
B. subtilis Medicinal_04 has the potential to serve as a multifunctional biocontrol agent and is capable of securing high-level disease suppression and optimizing plant productivity, offering a valuable toolkit for climate-smart, sustainable agriculture.
Misbah Ul Ain, Akhtar Hameed, S. Muzammil et al.· Frontiers in Microbiology· 0 citations
Ginseng (Panax ginseng), a traditional and valuable medicinal plant, is severely affected by rusty root rot caused by Ilyonectria robusta. Xylanase is hypothesized to play a key role in the pathogenicity of I. robusta. Through transcriptomic analysis during ginseng infection, the highly expressed xylanase gene IrXyn01...
Ya-Kun Zhang, Xiang-Kai Liu, Yi-Meng Wang et al.· Horticulturae· 0 citations
These findings demonstrate that both Trichoderma species function as multifunctional bioagents capable of simultaneously suppressing fungal and viral diseases while promoting tomato growth, with T. viride showing superior overall efficacy and strong potential for sustainable integrated disease management.
E. E. Ekpiken· FUDMA Journal of Agriculture...· 0 citations
Investigation of the biocontrol mechanisms of Bacillus velezensis L33a against V. dahliae JR2 in tomato suggests that L33a is a promising biocontrol strain for green management of tomato Verticillium wilt.
W. Jian, Yuan-Yuan Li, Yu Chen et al.· Microbiology Research· 0 citations
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