Aug 2026· Journal of Agricultural and Food Chemistry· Vol 74 32, pp.
25629-25643
· 0 citations· 43 references
Medicine
TL;DR
A novel bacterial strain, Enterobacter sp.
Abstract
Aflatoxin B1 is a potent Group I carcinogen that contaminates food and feed, necessitating efficient detoxification strategies. This study isolated a novel bacterial strain, Enterobacter sp. HNGD-822 from soil, which efficiently degrades AFB1. Genome analysis identified two novel multicopper oxidase (laccase) genes, EbMCO1 and EbMCO2. The recombinant enzymes were heterologously expressed, exhibiting optimal activity at 50 °C and pH 7.0, with strict Cu2+ dependence. Within 12 h, EbMCO1 degraded over 99% of AFB1, while EbMCO2 also showed high activity under the same conditions. Both enzymes primarily converted AFB1 into AFQ1 and epi-AFQ1, products that exhibited significantly reduced hepatotoxicity, developmental toxicity, and oxidative stress in transgenic zebrafish. Applied to artificially contaminated peanut meal matrices, EbMCO1 and EbMCO2 achieved degradation efficiencies of 89.35% and 82.66%, respectively, without altering nutritional quality. This work presents novel laccases with promising potential for the biocontrol of AFB1 in the feed and food industries.
This study provides the first genetic and biochemical evidence that a FadB-type enzyme participates in nicosulfuron catabolism, supporting sulfonylurea bridge cleavage and its potential for soil bioremediation.
Xian Wu, Kuo Liu, Yu-Lin Wu et al.· Journal of Hazardous Materia...· 0 citations
A thermostable laccase from Thermincola ferriacetica (Thfe-Lac) that is capable of transforming AFB1 in the absence of mediators is identified and represents efficient mediator-free laccase for decontamination of AFB1 in food and feed systems.
Bin-Bin Ouyang, Jiahao Gu, Wei Xu et al.· Food Research International· 0 citations
These findings provide a novel genomic and structural blueprint for aflatoxin biotransformation, highlighting the specific putative candidate enzymes, paaH and mhpB, for future investigations.
Rawan Muhammad Shady, A. Abdelkhalek, A. E. El Wahed et al.· Foods· 0 citations
Aflatoxin B1 (AFB1) is one of the most toxic mycotoxins, widely contaminating agricultural products and posing a serious threat to food safety and human health. Enzymatic degradation is considered a promising detoxification strategy due to its high efficiency, strong specificity, and lack of secondary pollution. AKR13B...
Qing-Wei Jiang, Juan Shen, Zhang-Hu Chen et al.· International Journal of Mol...· 0 citations
Abstract The Nostocales are filamentous cyanobacteria that include bloom-forming, nitrogen-fixing species with major ecological and biotechnological relevance. Owing to their metabolic diversity, nitrogen-fixing capabilities and nutritional profile, they hold potential for various biotechnological applications includin...
Gabriel D. Scoglio, David H. Green, Harry O. Jackson et al.· Microbial Genomics· 0 citations
Two enzymes from Bacillus velezensis HNGD-JQ06, laccase BVLac and superoxide dismutase BV2SOD were identified as broad-spectrum detoxification candidates and retained activity in peanut, corn, and wheat flour matrices, highlighting their potential for multi-mycotoxin biodetoxification.
