Jun 2026· Research in Plant Disease· Vol 32, pp. 157-165· 0 citations
TL;DR
Findings demonstrate that persimmon anthracnose in Korea is caused by multiple Colletotrichum species with clear genetic differentiation, and provides essential baseline information for disease diagnosis, epidemiological monitoring, and the development of resistant cultivars in future breeding programs.
Abstract
Persimmon anthracnose is a major disease affecting fruit quality and yield in persimmon-growing regions, yet information on the species diversity of causal pathogens in Korea remains limited. This study aimed to identify Colletotrichum species associated with anthracnose symptoms on persimmon fruits and to evaluate their pathogenicity. Sixteen fungal isolates were obtained from symptomatic fruits collected pear research center in Naju, Korea, and their morphological characteristics—colony features, conidial morphology, and appressoria— were examined. Multilocus sequence analysis using internal transcribed spacer, actin, β-tubulin, glyceraldehyde-3-phosphate dehydrogenase, chitin synthase I, calmodulin, the intergenic spacer region between Apn2 and Mat1-2-1 (ApMat) was performed to determine species identity and phylogenetic relationships. Phylogenetic analysis revealed that most isolates (87.5%) belonged to Colletotrichum horii. One isolate each of C. siamense and C. fructicola were also identified; notably, C. fructicola represents the first report of this species on persimmon in Korea. Pathogenicity tests showed that all three species induced typical sunken lesions on wounded fruits, and the inoculated pathogens were successfully re-isolated, fulfilling Koch’s postulates. These findings demonstrate that persimmon anthracnose in Korea is caused by multiple Colletotrichum species with clear genetic differentiation. The detection of C. fructicola suggests that the species spectrum of anthracnose pathogens may be broader than previously recognized. This study provides essential baseline information for disease diagnosis, epidemiological monitoring, and the development of resistant cultivars in future breeding programs.
The findings provide a theoretical foundation for accurate disease diagnosis, epidemiological surveillance, and the development of effective chemical control strategies for rubber plantations.
Zhiying Cai, Lili He, Li-Ming Dai et al.· Plants· 0 citations
This study aimed to investigate the occurrence of chili anthracnose, characterize the associated pathogen, and evaluate the antifungal potential of plant-derived products as sustainable management options in the Muong Khuong area, Lao Cai province. Field investigations showed that anthracnose was prevalent on both hot chili and sweet chili, with disease incidences of 50.50% and 70.48%, respectively, during the July survey. Anthracnose symptoms were observed on leaves and fruits of chili types. The pathogen was isolated from symptomatic tissues and characterized based on colony morphology, microscopic features, pathogenicity tests, and ITS sequence analysis. Re-inoculation on detached chili leaves and fruits reproduced anthracnose symptoms, confirming the pathogenicity of the isolate. BLASTn analysis of the ITS region revealed 100% sequence identity with species within the Colletotrichum gloeosporioides species complex. In vitro antifungal assays demonstrated that extracts from Piper betle, Elsholtzia ciliata, and Ocimum basilicum inhibited the pathogen's mycelial growth. Among them, Piper betle showed the strongest inhibitory effect (62.16% inhibition at 5 days after treatment), followed by Elsholtzia ciliata (23.91% inhibition at 5 days after treatment) and Ocimum basilicum (15.68% inhibition at 3 days after treatment). These findings support the potential use of plant-derived products for sustainable management of chili anthracnose.
Ong Xuan-Phong, Nguyen Huy-Thinh, Ngo Thi-Thuong et al.· CTU Journal of Innovation an...· 0 citations
Anthracnose is a fungal disease that severely affects mango (Mangifera indica L.) production globally. In Zimbabwe, mango production is a source of household nutrition and income from informal markets but there has been limited information on the fungal pathogens related to anthracnose in the main production areas. Lack of local baseline data limits proper diagnosis and the development of disease control measures. The main objective of this study was to morphologically characterize fungal isolates that were associated with anthracnose symptoms on mango fruits and leaves, which were collected from different orchards in the Mutoko District of Zimbabwe. Symptomatic samples from various trees were randomly collected in the different orchards. Pieces of tissue were taken from the edge of the lesions and surfaces sterilized, and were inoculation on potato dextrose agar. Colonies of the fungi that appeared were re-cultured to obtain pure culture. The isolates exhibited different colony morphology but mostly they produced grey to dark colonies of cottony to velvety textures. Based on morphology, the isolates were considered members of the Colletotrichum genus; nevertheless, species identification by molecular means was not performed. Through this research, the morphological characteristics of the anthracnose, associated Colletotrichum isolates from the Mutoko District were initially documented. This evidence calls for molecular and pathogenicity analyses to identify the species so that disease management can be effective.
Nyarai Chabikwa, Cleopas C. Chinheya, N. Mapope et al.· Journal of Tropical Plant Pr...· 0 citations
In vitro fungicide sensitivity assays, performed using the hyphal growth inhibition method against six common fungicides, demonstrated that Carbendazim was the most effective agent, indicating significant selectivity.
Fang Wu, Jing Tu, Zhu-xiang Liu et al.· Plant Disease· 0 citations
Fungal diseases are among the most persistent and underestimated threats to orchid cultivation, often causing severe decline if not detected early. In this study, leaf spot disease was recorded on two native orchid species, Dendrobium amabile and D. clavatum. To identify the causal agent, symptomatic leaves were collected and subjected to fungal isolation, pathogenicity testing, morphological characterization, and multi‑locus sequence analysis. Artificial inoculation reproduced typical leaf spot symptoms, confirming pathogenicity. Morphological traits of colonies and conidia indicated affiliation with the genus Exserohilum. Molecular analyses of the ITS, LSU, TEF1, and ACT gene regions further resolved the isolates as E. rostratum, with high sequence similarity among the representative strains. Minor morphological variation was noted, particularly in conidial dimensions, but overall consistency supported species‑level identification. This study establishes a new host association between E. rostratum and D. amabile and D. clavatum and demonstrates the effectiveness of combining morphological and molecular approaches for precise fungal identification. The generated multilocus dataset provides a useful reference for future studies on orchid pathogens and fungal taxonomy.
Thi Hong Phan Huynh, Dinh Don Le, Cách Tuyến Bùi· CTU Journal of Innovation an...· 0 citations
Results indicate that CLSD in Viet Nam is associated with a pathogen complex in which C. siamense is more pathogenic than P. microspora, while C. siamense grew most rapidly on potato-based media at 25–30°C.
Hieu Nguyen Don, Nhan Luan Doan, Thi Kim Uyen Nguyen· CTU Journal of Innovation an...· 0 citations
