Hepatoprotective Effects of Baccaurea motleyana Fruit Extract Against CCl4‐Induced Liver Injury in Rats: In Vivo Evaluation and GC–MS Phytochemical Profiling

Phytochemical Profiling and Preliminary Hepatoprotective Screening of Different Solvent Fractions of Elaeagnus parvifolia Fruit Extract Against Carbon Tetrachloride (CCl₄)–Induced Liver Injury in Mice

Evaluation of the Antioxidative and Hepatoprotective Activity of Ulva lactuca in a CCl4-Induced Liver Injury Model

Background: Ulva lactuca is abundant in bioactive components that provide antioxidative, anti-inflammatory and hepatoprotective properties against oxidative stress. Objectives: This study aimed to explore the nutritional composition, antioxidative potential and hepatoprotective efficacy of U. lactuca in a CCl4-induced liver injury model. Methods: Proximate analysis, phytochemical profiling (phenolic and flavonoid content, GC-MS and LC-MS) and antioxidant assays (DPPH and FRAP) were performed, followed by an in vivo efficacy trial. Hepatotoxicity was induced using CCl4, and U. lactuca methanolic extract (1000 mg/kg body weight) was administered for four weeks. Results: The proximate analysis demonstrated 10.5% ash, 10.83% protein and 39.43% fibre. GC-MS and LC-MS identified hexadecanoic acid methyl ester, gallic acid, quercetin, quercitrin, 4-hydroxybenzoic acid and syringic acid. TPC was higher in aqueous (2015.1 mgGAE/100 mL) and TFC in ethanolic (750.5 µgQE/mL) extract. Aqueous extract (170.1 ± 0.1 µmol/g) displayed superior reducing power while ethanolic extract (77.7%) exhibited the highest radical scavenging activity. U. lactuca promoted weight recovery, improved lipid profiles, decreased hepatic and oxidative stress biomarkers (p-value < 0.05), and restored liver histology. Conclusions: U. lactuca showed promising effects against sub-chronic liver injury, primarily due to its rich nutritional profile. Thus, its incorporation into the Pakistani diet may reduce disease and economic burden while supporting functional food and nutraceutical industries.

Antioxidant-Mediated Hepatoprotective Activity of Curcuma pseudo-montana Rhizome Extracts Against D-Galactosamine-Induced Hepatic Injury in Rats

Background: Liver diseases remain a major global health problem, and currently available therapies are often associated with limitations and adverse effects. Medicinal plants rich in antioxidant phytoconstituents have emerged as promising alternatives for the management of hepatic disorders. Curcuma pseudo-montana is traditionally used in folk medicine for the treatment of various ailments, including liver-related disorders. Objective: The present study was undertaken to evaluate the hepatoprotective effect of 50% methanolic extract of Curcuma pseudo-montana rhizomes against D-galactosamine-induced hepatic injury in rats. Materials and Methods: Hepatic injury was induced in Wistar albino rats using D-galactosamine (600 mg/kg, i.p.). Animals were treated with 50% methanolic extract of Curcuma pseudo-montana rhizomes at doses of 200 and 400 mg/kg body weight. Silymarin (100 mg/kg) was used as the standard hepatoprotective drug. Serum biochemical markers including SGOT, SGPT, ALP, bilirubin, and total protein were evaluated. Antioxidant parameters such as catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), and malondialdehyde (MDA) were estimated. Histopathological examination of liver tissues was also performed. Results: D-galactosamine administration produced significant hepatic injury characterized by elevated serum SGOT, SGPT, ALP, and bilirubin levels along with a reduction in total protein content. Oxidative stress was evidenced by decreased CAT, SOD, and GSH levels and increased MDA concentration. Treatment with the 50% methanolic extract significantly restored liver function markers and antioxidant enzyme levels in a dose-dependent manner. The higher dose (400 mg/kg) demonstrated greater hepatoprotective activity and significantly reduced lipid peroxidation. Histopathological examination confirmed marked protection against hepatocellular degeneration, necrosis, and inflammatory infiltration, with near-normal hepatic architecture observed in the high-dose treatment group. Conclusion: The findings demonstrate that the 50% methanolic extract of Curcuma pseudo-montana rhizomes possesses significant hepatoprotective activity against D-galactosamine-induced liver injury. The protective effect may be attributed to its antioxidant and anti-inflammatory properties, supporting the traditional use of the plant in liver disorders.

Antioxidant and anti-inflammatory effects of polyphenol-rich Illicium verum extract on lipopolysaccharide-induced liver injury in rats

Pretreatment with PEIV significantly mitigated effects of LPS-induced liver injury by regulating biochemical parameters, reducing oxidative stress and inflammation, and preserving liver architecture, suggesting its potential as a natural therapeutic agent for liver diseases.

Polygonatum odoratum extract ameliorates acetaminophen-induced acute liver injury by modulating the gut-liver axis and remodeling microbial and ceramide metabolism to suppress hepatic ferroptosis: insights from multi-omics

Context Drug-induced liver injury remains a major clinical challenge due to the lack of effective therapeutic options. Polygonatum odoratum extract (PO), derived from the traditional medicinal and edible plant Polygonatum odoratum (Mill.) Druce, has long been used in folk practices in forms such as herbal soups and tea infusions. However, its protective effect against acetaminophen (APAP)-induced liver injury has not yet been investigated. Objective This study evaluated the preventive effects of PO against APAP-induced acute liver injury in mice. Materials and Methods An APAP-induced acute liver injury model was established in C57BL/6 mice. The hepatoprotective effects of PO were validated via histopathological analysis, oxidative stress assays,and serum biomarkers Multi-omics approaches, including 16 S rDNA sequencing, transcriptomics, and network pharmacology, were integrated with molecular biology techniques and pseudo-germ-free (PGF) mouse models to elucidate the underlying mechanisms. Results PO significantly alleviated hepatic histopathological damage, oxidative stress, and reduced serum ALT, AST, and LDH. PO remodeling gut microbiota, mitigated colonic inflammation and oxidative stress, and improved intestinal morphology, effects abolished by microbiota depletion. Mechanistically, PO maybe inhibited hepatic ferroptosis via the gut microbiota–ceramide axis, evidenced by upregulation of GPX4 and FSP1, NRF2/KEAP1 pathway activation, downregulation of ACSL4, TFRC/TFR2, and DUOX2, restored iron homeostasis, and decreased lipid peroxidation and ROS. In vitro, PO demonstrated anti-ferroptotic effects in APAP- or RSL3-treated AML12 cells. Discussion and Conclusion PO confers hepatoprotection via the gut–liver axis, which is associated with changes in ceramide metabolism and ferroptosis-related markers.

EVALUATION OF THE HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITIES OF HYDROALCOHOLIC EXTRACT OF IMPATIENS BALSAMINA LINN. LEAVES AGAINST DICLOFENAC-INDUCED HEPATOTOXICITY IN RATS

Drug-induced liver injury is a major health concern and represents one of the most common causes of acute liver failure. Diclofenac, a widely prescribed non-steroidal anti-inflammatory drug (NSAID), is known to induce hepatotoxicity through oxidative stress, mitochondrial dysfunction, and the formation of reactive metabolites. Natural products with antioxidant properties have gained attention as potential hepatoprotective agents. Impatiens balsamina Linn. is a medicinal plant traditionally used for various therapeutic purposes and is rich in flavonoids, phenolic compounds, tannins, and other bioactive constituents with potent antioxidant activity. The present study was undertaken to evaluate the hepatoprotective activity of the hydroalcoholic extract of Impatiens balsamina leaves (HAEIB) against diclofenac-induced hepatotoxicity in experimental rats. HAEIB treatment significantly restored altered biochemical parameters, increased GSH and SOD levels, and reduced LPO levels compared with the diclofenac-treated group, indicating attenuation of oxidative stress. Histopathological examination revealed marked protection against hepatic degeneration, necrosis, and inflammatory cell infiltration. HAEIB at a dose of 400 mg/kg demonstrated hepatoprotective activity comparable to that of the standard drug silymarin. These findings suggest that the hepatoprotective effect of HAEIB may be attributed to its antioxidant phytoconstituents and support its potential use as a natural hepatoprotective agent.