Microgreens in controlled environment agriculture: a comprehensive review of nutritional optimization, agronomic innovations, and postharvest sustainability.

Biochar in Controlled Environment Agriculture: Applications in Hydroponics, Vertical Farming, and Soilless Cultivation

Controlled environment agriculture (CEA), including hydroponics, vertical farming (VF), and soilless cultivation, is expanding rapidly as food production shifts toward resource-efficient and climate-resilient systems. However, conventional substrates such as rockwool, peat, coco coir, and perlite present limitations related to nutrient buffering, structural stability, and environmental sustainability. Biochar has emerged as a promising alternative substrate component due to its porous structure, surface functionality, and ability to modify root-zone conditions. This review synthesizes current knowledge on the role of biochar in controlled cultivation systems, focusing on its physicochemical properties, substrate interactions, and plant physiological responses. Biochar incorporation influences water retention, aeration, nutrient availability, and microbial activity within confined root environments, thereby improving root architecture, photosynthetic performance, crop quality, and plant uniformity. Applications across hydroponic, VF, and soilless cultivation systems demonstrate improved moisture regulation, nutrient buffering, and substrate stability. Biochar interactions with conventional media such as coco peat, perlite, and peat moss further highlight its role in engineered growing substrates. Despite these advantages, challenges remain, including feedstock variability, pH and electrical conductivity effects, lack of standardized specifications, and limited long-term performance data in recirculating systems. Emerging research areas such as engineered biochar, nano-biochar, microbial integration, and precision cultivation technologies offer opportunities to optimize biochar performance in controlled environments. Overall, biochar represents a versatile and sustainable substrate component for CEA, with potential to enhance crop productivity, substrate durability, and resource efficiency. Future research should focus on material standardization, system-specific optimization, and large-scale validation to support commercial adoption.

Wheat Microgreens for Global Food Security: Novel Interventions for Sustainable Production and Functional Enrichment.

Wheat (Triticum aestivum L.) microgreens have emerged as nutrient-dense functional foods with significant potential to address malnutrition, lifestyle-related disorders, and the growing demand for sustainable diets. Rich in vitamins, minerals, flavonoids, carotenoids, and phenolic compounds, they demonstrate diverse bioactivities including antioxidant, anti-inflammatory, antidiabetic, anticancer, and cardioprotective effects. Their short growth cycle, minimal input requirements, and adaptability to hydroponic and vertical farming systems further position them as resource-efficient crops for future food security and for space cropping. Despite these advantages, challenges such as limited shelf life, microbial contamination, lack of standardized post-harvest practices, and low consumer awareness restrict their large-scale commercialization. This review consolidates current knowledge on the nutritional, functional, and therapeutic relevance of wheat microgreens while exploring optimized growing conditions, biofortification strategies, genetic improvement, and post-harvest management to enhance phytochemical accumulation and nutritional quality. Advances in omics technologies, marker-assisted selection, and genome editing tools such as CRISPR/Cas9 are opening new avenues for improving resilience, delaying senescence, and tailoring nutritional traits. Parallel developments in minimal processing, modified atmosphere packaging, edible coatings, and smart packaging systems offer promising solutions to extend freshness and ensure food safety. In addition, the economic and environmental benefits of wheat microgreens highlight their potential in premium markets and sustainable farming models, including controlled-environment agriculture and technologies like digital twins. By integrating nutritional science, biotechnology, and food system perspectives, this review identifies key challenges and opportunities to position wheat microgreens as a sustainable, health-promoting superfood for future diets.

Agronomic management enhances nutritional quality and functional food potential of purslane (Portulaca oleracea L.): a review

The growing global challenges of food insecurity posed by climate change, and micronutrient deficiencies have intensified interest in climate resilient, nutrient-dense functional foods. Purslane (Portulaca oleracea L.) is a promising candidate owing to its exceptional nutritional profile and adaptability to harsh agroecological conditions. This review provides a comprehensive and critical synthesis of existing literature on the agronomic management, nutritional composition, and functional food potential of purslane, with particular emphasis on its omega-3 fatty acid content and the role of nitrogen and mineral nutrition. The review reveals that purslane is a rich source of alpha-linolenic acid (ALA), vitamins (C and E), carotenoids, minerals, and diverse bioactive compounds with antioxidant and anti-inflammatory properties. However, its nutritional quality is highly variable and influenced by genotype, environmental conditions, cultivation systems, and nutrient management strategies. Nitrogen plays a dual role of enhancing biomass production while exerting complex, compound-specific effects on nutrient density, often leading to trade-offs between yield and quality. The novelty of this study lies in its integrated, multi-dimensional perspective that links agronomy, plant physiology, and nutritional science, moving beyond fragmented analyses in existing literature. It highlights the importance of genotype-by-environment interactions and introduces the concept of “nutritional efficiency” as a more appropriate framework than yield-focused optimization. This study contributes to knowledge by identifying critical gaps and proposing a systems-based approach for optimizing purslane as a sustainable, plant-based source of omega-3 fatty acids and functional nutrients, particularly in resource-constrained and climate-vulnerable regions.

Trends in biofertilisation and agroecological practices for the sustainable enhancement of CAM plants growth in arid environment: the case of Opuntia ficus-indica

Crop Biofortification for Sustainable Food Systems: An Integrative Review of Soil Processes, Plant Physiology and Molecular Approaches

Micronutrient deficiencies, collectively known as hidden hunger, affect more than two billion people worldwide and remain a major challenge for sustainable agriculture, global food security and human nutrition. Crop biofortification has emerged as a sustainable agricultural strategy to enhance the concentration and bioavailability of essential micronutrients in edible plant tissues while reducing reliance on post-harvest fortification and dietary supplementation. This review provides an integrated analysis of the soil, plant physiological, agronomic and molecular processes governing biofortification efficiency in agricultural systems. Particular emphasis is placed on how soil formation, mineralogy, nutrient speciation, organic matter and rhizosphere interactions regulate micronutrient availability, root uptake, translocation and accumulation in crops. The review further examines plant physiological mechanisms involved in nutrient acquisition and partitioning, together with the contribution of beneficial microorganisms, precision agriculture and digital technologies to improving nutrient-use efficiency under diverse agricultural conditions. Conventional breeding, agronomic biofortification, transgenic approaches and genome-editing technologies are critically evaluated as complementary strategies for developing nutrient-enriched and climate-resilient crop varieties. Particular attention is also given to nutrient bioavailability, post-harvest stability and consumer acceptance, which ultimately determine the nutritional effectiveness of biofortified crops. Furthermore, the review discusses how climate change modifies soil properties, plant physiology and crop productivity, thereby influencing micronutrient availability, nutrient accumulation and the long-term effectiveness of biofortification programmes. By integrating advances in soil science, plant physiology, agronomy and molecular biology, this review identifies current challenges, knowledge gaps and future research priorities for developing resilient biofortification strategies capable of supporting sustainable agricultural systems and improving global nutritional security.

Biostimulants for Sustainable and Resilient Agriculture

The growing pressure exerted by global food demand, combined with the excessive use of chemical and synthetic inputs, is prompting the agricultural sector to seek innovative and sustainable solutions to improve, or at least maintain, crop yields in a context of increased abiotic stress linked to climate change. Among the promising approaches, biostimulants are attracting growing interest, particularly those derived from natural sources such as seaweed extracts, humic acids, and beneficial microorganisms. These products work through various mechanisms, including osmotic regulation, activation of antioxidant systems, stimulation of root growth, and improvement of nutrient absorption. Many recent research and review articles have explored the optimal combinations of raw materials, formulation processes, target crops, and environmental conditions to maximize beneficial effects on plant growth, soil health, and tolerance to abiotic stresses. As a result, a growing range of commercial products is emerging, with diverse chemical compositions, formulations, and modes of application. However, the precise relationships between the biochemical composition of biostimulants and their physiological effects remain poorly understood, suggesting a key role for molecular synergies. This review provides a concise overview of recent advances in biostimulant research and their potential to enhance food security by improving crop resilience in the context of climate change.