Aug 2026· Horticulturae· Vol 12, pp. 1027· 0 citations· 46 references
TL;DR
Overall, strain Y107 promoted lingonberry growth through stable colonization, enhanced nutrient cycling, promoted lingonberry growth, and altered the expression patterns of genes involved in photosynthesis-related pathways.
Abstract
Lingonberry (Vaccinium vitis-idaea L.) has a weak root system and limited nutrient acquisition capacity. Plant growth-promoting rhizobacteria (PGPR) can promote plant growth by regulating rhizosphere nutrient cycling and host metabolism, but the underlying mechanisms in Ericaceae remain unclear. In this study, the strain Microbacterium sp. Y107 was isolated from the rhizosphere of lingonberry. Using growth-promoting assays (with 9 independent biological replicates, each consisting of 3 plants per bottle as the experimental unit, an inoculum density of OD600 = 1.0, a cultivation period of 60 days, and whole-plant harvesting), GC–MS-based metabolite analysis, axenic coculture, analyses of the rhizosphere environment and gene expression, transcriptome sequencing (3 biological replicates per treatment), and qRT–PCR validation, we systematically evaluated the effects of strain Y107 on lingonberry growth, rhizosphere conditions, and host gene expression. CK (control) plants were defined as those inoculated with sterile LB liquid medium. Pearson correlation analysis was also used to assess the relationships among plant growth, soil traits, and physiological indices. Compared with the CK treatment, strain Y107 significantly promoted lingonberry growth (p < 0.05), increasing the fresh weight of the roots by 157.65% and the whole-plant fresh weight by 33.85%, respectively, and increasing peroxidase (POD) activity by 61.11%, while reducing the contents of reducing sugars and proline. Strain Y107 stably colonized the lingonberry root surface and formed biofilms. Y107 treatment resulted in detectable levels of soil microbial biomass carbon and nitrogen, indicating successful microbial colonization. The ammonium nitrogen content significantly increased, increasing nitrogen availability and promoting root nutrient uptake. Transcriptome analysis revealed that the expression of genes enriched in photosynthesis-related pathways, as well as those involved in carbon and nitrogen metabolism and redox regulation, was altered in strain Y107, which upregulated key photosynthesis-related genes such as psbP and ndhU and significantly enriched the cytosolic ribosomal large subunit gene set in a negative direction. Correlation analysis further revealed that strain Y107 optimized the association between lingonberry growth and soil nutrients. Overall, strain Y107 promoted lingonberry growth through stable colonization, enhanced nutrient cycling, promoted lingonberry growth, and altered the expression patterns of genes involved in photosynthesis-related pathways.
Objective Continuous cropping obstacles and heavy chemical fertilizer use constrain Radix serratulae chinensis cultivation. This study evaluates the optimal concentration of plant growth-promoting rhizobacterium Bacillus amyloliquefaciens ART9 and investigates its underlying mechanisms via rhizosphere microbiome and host transcriptome analyses. Methods Seedlings were treated with ART9 suspensions at OD600 of 0.4, 0.6, and 1.0. Growth parameters, soil nutrient contents, rhizosphere bacterial communities, and root transcriptomic profiles were assessed to compare treatment effects. Results The OD600 0.6 treatment yielded the best performance, increasing plant height, stem diameter, and SPAD value by 48.56%, 14.09%, and 12.42%, respectively, over the control, and raising soil available nitrogen, phosphorus, and potassium by 177%, 179%, and 168%. Rhizosphere microbiota were significantly enriched with beneficial genera, notably Bacillus, Pseudomonas, and Sphingomonas. Transcriptomics revealed prominent enrichment in phenylpropanoid biosynthesis (map00940), plant hormone signal transduction (map04075), and circadian rhythm–plant (map04712) pathways. Upregulated genes included JAZ, ABF, PYL, PP2C, SPA1_2, and HY5, indicating coordinated regulation of hormone and light signaling. Conclusion ART9 systematically promotes growth of Radix serratulae chinensis by reshaping the rhizosphere microbiome and modulating hormone/light signaling pathways, supporting its development as a sustainable biofertilizer for medicinal plant production.
Jiaxin Wang, Jinjie Xu, Pengzhong Fu et al.· Frontiers in Plant Science· 0 citations
This study introduces L. aquatilis strain MC3 as an emerging candidate for bioinoculant development and one of the first reports for identification of L. aquatilis as multifunctional PGPR from Himalayan ecosystems.
S. Devi, Riya Chandel, D. Thakur et al.· Frontiers in Systems Biology· 0 citations
The potential of P1 as a promising bioinoculant candidate for sustainable agriculture in the potato sector is demonstrated and the genome lacked major virulence factors and antimicrobial traits, supporting the non-pathogenic nature of the P1 strain.
Poonam Patel, K. Raval, Satyamitra Shekh et al.· Frontiers in Microbiology· 0 citations
Bioprospecting of plant growth-promoting bacteria enables the identification of beneficial microbial resources with significant potential for sustainable agricultural applications. In this context, strain TRQ48 was isolated from a commercial field of wheat (Triticum turgidum L. subsp. durum) located in the Yaqui Valley, Mexico, with the aim of exploring its plant growth-promoting potential. The draft genome sequence presented a genomic size of 2,777,016 bp, 32.5% G + C content, 665,763 bp N50, 2 L50, and 19 contigs. Taxonomic affiliation demonstrated that strain TRQ48 belonged to Mammaliicoccus sciuri. Genome annotation identified 2756 coding DNA sequences (CDS) distributed into 259 subsystems, highlighting CDS associated with iron acquisition and metabolism, stress response, and virulence, disease, and defense, among others. Metabolic assays reflect the strain’s capacity to produce siderophores and auxins, relating these positive traits to significantly (p ≤ 0.05) promote growth of wheat shoot length (7.09%) and root and shoot dry weight (75% and 18.43%) compared to uninoculated wheat plants. Biosafety testing indicated that the strain is susceptible to commonly used antibiotics and lacks clinically relevant resistance profiles, matching genomic analysis, which did not reveal any critical virulence factors. Thus, although the presented results show that M. sciuri TRQ48 is a promising beneficial biosafe strain, further studies are still needed to evaluate its performance under agro-ecosystems at commercial levels.
América Lizeth Sánchez-Zúñiga, Luis Alberto González-Vázquez, Alina Escalante-Beltrán et al.· Agronomy· 0 citations
Rhizobium spp. inoculation is a strategy to promote plant growth and enhance drought tolerance, particularly in semiarid environments. This study evaluated the morphological and physiological responses of Mimosa tenuiflora (Willd.) seedlings inoculated with Rhizobium spp. under different water contents. Seeds were collected from 12 naturally growing mother trees (Patos, Paraíba, Brazil). No specific genotype or cultivar was used. A completely randomized experiment was conducted in a 5 × 2 factorial design with four replicates, comprising five treatments (inoculation with three Rhizobium spp. strains: CA19PT, RO19PT, and MK17PT; nitrogen fertilization; and a non-inoculated control) under two water-content levels (40% and 80% of pot capacity). M. tenuiflora seedlings were grown in 3 dm³ pots for 90 days, after which growth, biomass production, nodulation, relative water content (RWC), and gas exchange parameters were evaluated. The results demonstrated that physiological variables were influenced by the treatments and water availability, whereas growth variables were affected only by water availability. Among the evaluated treatments, inoculation with strain CA19PT showed physiological responses comparable to those observed under nitrogen fertilization. Even under low water availability (40% pc, seedlings maintained adequate hydration levels, with an increase in root biomass. Inoculation with selected Rhizobium isolates, particularly CA19PT, improved the physiological performance of M. tenuiflora seedlings under contrasting water-content conditions. Physiological responses observed in seedlings inoculated with CA19PT were comparable to those obtained with nitrogen fertilization. Additionally, seedlings grown under higher water content (80% pc exhibit better overall growth, whereas reducing water content to 40% pcstimulates root growth.
Erika Rayra Lima Nonato, Carlos Luiz da Silva, Moema Barbosa de Sousa et al.· Discover Plants· 0 citations