An Osteogenic Porous Silica‐Incorporated Fibrous Composite for Bone Tissue Engineering Applications: Fabrication, Characterization, and Biological Evaluation
TL;DR
Good hemocompatibility, cytocompatibility, bioactivity, and calcium mineralization ability, along with an increased ALP activity after 14 days, supported the osteogenic potential of the scaffold in BTE.
Abstract
This study focuses on fabricating SiO 2 ‐incorporated PVA/PVP/SF fiber composite, which holds significant potential for BTE applications due to its integrated bioactivity, biocompatibility, and porosity. SiO 2 was synthesized through the sol–gel route, and the fibrous scaffold via electrospinning. X‐ray diffraction (XRD), Fourier transform ‐ infrared (FT‐IR) and field‐emission scanning electron microscopy (FE‐SEM) confirmed the structural and morphological characteristics of the scaffolds. The porosity reached 99% within 48 h, ensuring good cell attachment and proliferation. Scaffolds showed an adequate hydrophilicity and a tensile strength of 8.75 MPa, with 80.2% elongation at break. The antibacterial activity of SiO 2 against Escherichia coli (13 ± 0.51 mm) and Staphylococcus aureus (11.8 ± 0.75 mm) was significantly improved in fibrous composites with the highest inhibition zones of 30.3 ± 1.03 and 28.0 ± 0.96 mm, respectively. Good hemocompatibility, cytocompatibility, bioactivity, and calcium mineralization ability, along with an increased ALP activity after 14 days, supported the osteogenic potential of the scaffold in BTE.