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VINE-seq provides a robust, reproducible workflow for the enrichment and high-resolution profiling of vascular, perivascular, and immune cells from fresh or frozen human and mouse brain tissue and optimized extraction of nuclei from purified vessels using enzymatic digestion.
A comprehensive cerebrovascular cell atlas encompassing 314,535 transcriptomes is constructed that captures the arteriovenous axis and defines consensus cell states and identifies ensemble-specific susceptibilities and candidate therapeutic targets across neurological diseases, including small vessel disease and stroke...
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