VINE-seq provides a robust, reproducible workflow for the enrichment and high-resolution profiling of vascular, perivascular, and immune cells from fresh or frozen human and mouse brain tissue and optimized extraction of nuclei from purified vessels using enzymatic digestion.
This review systematically integrates current evidence from sc/snRNA-seq studies, with a primary focus on brain-intrinsic mechanisms after ischemic stroke, to provide a structured synthesis of how sc/snRNA-seq has advanced the understanding of ischemic stroke biology.
Chang-Qing Mu, Yu-Chuan Ding, Alexander Weiss et al.· Frontiers in Neurology· 0 citations
The clinical translational trajectory is delineated, illustrating how aberrant signatures are verified in cerebrospinal fluid and validated in plasma to support precision medicine and arguing for the necessity of "fused" multi-omics integration and Artificial Intelligence to decode the non-linear molecular logic of bra...
Arup Acharjee, Nitika Rai, Akash A. Patel et al.· Biochimica et Biophysica Act...· 0 citations
This work used isolated brain vascular fragments to study the blood–brain barrier and applied a combination of deep bulk proteomic analysis, a proteomic ruler approach, and scRNA-seq, assuming a high within-gene correlation between mRNA and protein.
Li-Qun He, Henrik J. Johansson, E. Vázquez-Liébanas et al.· Proceedings of the National...· 0 citations
Brain aging is a major risk factor for cerebrovascular and neurodegenerative diseases, yet continuous cell-type changes across the human lifespan remain incompletely defined. Here, we integrate 15 prefrontal cortex (PFC) snRNA-seq datasets from 158 neurologically healthy donors (19–101 years) to construct an atlas of...
Rui-Ze Niu, Meng-Yuan Zhang, Zhi-Lan Yang et al.· npj Aging· 0 citations
Interindividual heterogeneity in Alzheimer’s disease (AD) remains poorly understood, as disparate single-cell studies leave it unclear whether findings reflect shared architecture or dataset-specific idiosyncrasies. Here, we present panAD, a transcriptomic atlas of >3 million nuclei from 791 individuals across 13 studi...
Negin Rahimzadeh, S. Morabito, S. Khullar et al.· bioRxiv· 0 citations
Neurodegenerative and neuropsychiatric diseases impose a considerable societal and public health burden. However, our understanding of the molecular mechanisms underlying these highly complex conditions remains limited1,2. Here, to gain deeper insights into the aetiology of different brain diseases, we used specimens f...
Donghoon Lee, Mikaela Koutrouli, Nicolas Y. Masse et al.· Nature· 5 citations
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