Nucleofection-Based CRISPR/Cas Delivery in Human T Cells for Immunotherapy Applications.
Electroporation-based delivery of CRISPR/Cas systems has emerged as a powerful and versatile approach for gene editing in primary human T cells, enabling efficient, transient, and nonviral modification while minimizing genomic integration risks. This chapter focuses on the principles and practical implementation of ele...
María Ortiz-Bueno, Alejandro Millán-López, Kornel Labun et al.
· Methods in molecular biology · 0 citations