Ana Cuervo

Decoding the Cellular Response to Methylthioadenosine Uncovers Novel Targets for Chronic Liver Disease

Progressive familial intrahepatic cholestasis type 3 (PFIC3), caused by MDR3 deficiency (MDR2 in mice), is a lethal pediatric liver disease characterized by early-onset persistent cholestasis, progressive fibrosis, cirrhosis, and eventual liver failure. MDR3 is a phosphatidylcholine transporter located in the canalicul...

The Open Conformation of CCT: How to Achieve High Resolution Using CryoEM.

CryoEM Strategies for Elucidating the Closed State Architecture of CCT.

This study investigates the eukaryotic chaperonin CCT, a ~1 MDa hetero-oligomeric complex essential for the folding of key substrates such as actin, tubulin, and WD40 family members and demonstrates that subunit assignment and atomic modeling are achievable by exploiting subunit-intrinsic structural features alone.