CryoEM Strategies for Elucidating the Closed State Architecture of CCT.

The Open Conformation of CCT: How to Achieve High Resolution Using CryoEM.

Cryo-tides: synthetic peptides for cryo-EM grid preparation of multi-subunit protein complexes sensitive to the air-water interface

Cryogenic electron microscopy (cryo-EM) has enabled rapid progress in structural biology throughout the last decade. However, cryo-EM sample preparation still represents a major bottleneck in high-resolution structure determination. Many proteins and macromolecular complexes interact with the air-water interface during...

Scaffold engineering enables high-resolution cryo-EM analysis of thiamine pyrophosphate riboswitch architecture and dynamics.

Recent advances in cryoelectron tomography and applications to parasitology.

This review highlights the range of resolutions and cellular structures accessible by cryo-ET, and the kinds of biological insights that may be obtained, using eukaryotic parasites as case studies, and combines with orthogonal techniques.

Subtomogram Averaging of Membrane Complexes in Organelle-Derived Vesicles.

Design and Optimization of BRIL Fusion Constructs for Membrane Protein Cryo-EM Studies.

Structural determination of small membrane proteins by cryo-electron microscopy (cryo-EM) remains inherently challenging due to their low molecular weight, conformational heterogeneity, and lack of prominent structural features for reliable particle alignment. In this chapter, we describe how to design and perform biop...