CryoEM Strategies for Elucidating the Closed State Architecture of CCT.
This study investigates the eukaryotic chaperonin CCT, a ~1 MDa hetero-oligomeric complex essential for the folding of key substrates such as actin, tubulin, and WD40 family members and demonstrates that subunit assignment and atomic modeling are achievable by exploiting subunit-intrinsic structural features alone.