Author

B. Kloss

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Open access Sep 2026

A molecular safeguard in Escherichia coli Braun’s lipoprotein (Lpp) biogenesis

ABSTRACT Braun’s lipoprotein (Lpp) is the most abundant protein present in Escherichia coli. Lpp is an outer membrane (OM) lipoprotein that contributes to the mechanical integrity of the cell envelope by tethering the OM to the underlying peptidoglycan (PGN). It has long been known that Lpp accumulation at the inner me...

Emily J. LaMarre, Makayla R. Braunlin, B. Kloss et al. · mBio · 0 citations

2026

Expression and Purification of Integral Membrane Enzymes for Functional and Structural Studies.

Together, the methods described here demonstrate that careful selection via thorough screening, and optimization of expression and purification conditions, are an invaluable investment of time, ultimately yielding the best target not only for successful high-resolution structural analyses-X-ray crystallography and cryo...

B. Kloss, C. Ardiccioni, V. Petrou et al. · Methods in molecular biology · 0 citations