Expression and Purification of Integral Membrane Enzymes for Functional and Structural Studies.

Expression, Purification, and Structural Characterization of Membrane Proteins: A Case Study of the ABC Transporter MsbA.

This chapter outlines a detailed workflow for the expression, purification, and structural analysis of the ABC transporter MsbA from Pseudomonas aeruginosa (PaMsbA), which exemplifies how biochemical optimization, native mass spectrometry, and cryo-electron microscopy (cryo-EM) can be integrated to investigate membrane...

Isolation of AtSWEET Membrane Proteins from Arabidopsis Leaves for Host-Pathogen Interaction Studies.

The current protocol outlines a method for membrane purification utilizing ultracentrifugation and detergents, which facilitates the isolation of membrane proteins in their native conformational state.

A generalisable method for the purification and biophysical characterisation of bacterial membrane receptors

A generalizable workflow for producing soluble receptor domains from membrane proteins is established and validated HasR constructs suitable for downstream ligand-screening applications, including aptamer and nanobody discovery.

Purification and Sonication-Assisted Cryo-EM Grid Preparation of TolC.

Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.

Transmembrane signalling by membrane proteins is essential for physiological processes and disease states, making these proteins key targets for drug development. Structural biology provides unique insights into the mechanisms of membrane protein function and dysfunction caused by mutations. Most high-resolution membra...

Effects of Detergent Selection on Stability of a Recombinant Integral Membrane Protein

Clear trade-offs are revealed between detergents that excel in membrane solubilization and those that better preserve conformational stability, with no single detergent outperforming across all metrics.