Author

Blandina I. Oliveira Esteves

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#gene editing Open access Oct 2026

Genetically modified primary airway-epithelial cell culture models to study virus-host interactions of emerging respiratory viruses

This chapter describes the generation of knockout (KO) AECs using engineered virus-like particles (eVLPs), which transiently deliver the Cas9–gRNA ribonucleoprotein (RNP) complex into target cells, enabling efficient genome editing of any gene of interest.

Karen Stevers, Sarah Jordi, Blandina I. Oliveira Esteves et al. · bioRxiv · 1 citation

#gene editing Open access Oct 2026

Genetic modification of primary airway epithelial cell cultures defines MX1 as a barrier for zoonotic influenza A viruses in the human respiratory epithelium

The antiviral innate immune response is a major species barrier that emerging viruses must overcome to infect and transmit efficiently in new hosts. In humans, the interferon-stimulated MX1 gene encodes the antiviral MX1 protein, which restricts zoonotic influenza A viruses (IAVs). Resistance to MX1 is considered a key...

Karen Stevers, Blandina I. Oliveira Esteves, Étori Aguiar Moreira et al. · bioRxiv · 0 citations