Stability analysis of the complete single mutant library of a protein domain
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Automated techniques for the complete site-directed mutagenesis and stability analysis of protein domains
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MOP1 affects DNA methylation, nucleosome occupancy, histone modifications and chromatin conformation at the b1 allele involved in paramutation
The results uncover an intricate interplay between active and silent epigenetic features present at the b1 locus in a mop1 background, resulting in elevated expression of B’, concluding that MOP1 is essential to maintain the B’ silent chromatin state.
M. Louwers, M. Haring, R. Bader et al.
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