Chunhai Fan

DNA Nanoruler-Assisted Linearization for Long-Chain Nucleic Acids Analysis.

Long-chain nucleic acids (LCNAs) are increasingly important as diagnostic biomarkers, yet their quantitative detection is often hindered by compact conformations that limit probe accessibility. Here, we report a DNA nanoruler-assisted linearization strategy that mechanically regulates LCNA conformation by employing a r...

Programming Hydrophobic Nanocavity of DNA Frameworks for Ultrastable Fluorescent Imaging.

Fluorescent proteins (FPs) are widely used in biological imaging, yet their limited programmability restricts adaptability for advanced nanoscale applications. Synthetic molecular containers offer designable cavities but struggle to simultaneously achieve large dimensions, biocompatible interfaces, and precise microenv...

Decoding mechanoregulation in immunological synapses using biomimetic artificial cells

Innate Immunity of Framework Nucleic Acids.

This Account reviews efforts to develop framework nucleic acids (FNAs) as a platform with modulable innate immunostimulation for biomedical applications in live cells and in vivo, and envision FNAs as intelligent tools for precision immunomodulation, bridging nanoscale design with immunological outcomes to advance pers...

Direct Cytosolic Delivery of Amphiphilic Framework Nucleic Acids for RNA Interference.

A class of amphiphilic framework nucleic acids (ampFNAs) that enable direct cytosolic delivery involving energy-independent traversal of lipid membranes and establishes ampFNAs as a programmable, efficient, and biocompatible platform for the development of next‑generation smart nucleic acid delivery machines for precis...

Direct Visualization of Orientation Competition in Protein Epitaxial Crystallization.

In situ atomic force microscopy is used to reveal the two-dimensional crystallization dynamics of streptavidin on muscovite mica with single-molecule resolution, establishing a paradigm of domain coarsening in biomolecular epitaxy, providing a solid foundation for the rational design of functional bio-nano interfaces.