protein folding

A Comprehensive Survey of Crystal Structural Prototypes: Construction, Classification, and Applications

Crystal structural prototypes describe recurring atomic arrangements across chemically distinct materials. Prototype libraries are built through crystallographic curation, standardization, geometric matching, local-environment clustering, and periodic-graph analysis, and support chemical substitution, derivative enumer...

Influences of body protein and body fat on oestrus of yearling caribou

Abstract Age of primiparity is a critical demographic parameter that influences lifetime reproductive success and fitness. Caribou (Rangifer tarandus) exhibit variation in age of primiparity but generally are considered sexually immature (i.e. do not ovulate or achieve pregnancy) until they are ≥2.5 years old, unless n...

Food-Relevant Polycyclic Aromatic Hydrocarbons Disrupt Intestinal Barrier and Xenobiotic Metabolism in Mouse, Pig, and Human Epithelial Cells.

Because BaP may not fully account for the toxicity of all PAHs, this study provides relevant quantitative benchmarks for intestinal toxicity, advancing knowledge on current PAH exposure risk assessment.

Integration of blood‐based biomarkers and genomic variants identifies individuals with mild cognitive impairment who benefit from multidomain intervention

The findings suggest that BBMs, potentially in combination with genetic information, may help identify individuals with a more progressive AD‐related biological profile who may derive greater benefit from multidomain intervention.

Optimized Deep Knowledge Distillation Framework for Large-Scale Virus-Host Protein Interaction Prediction.

The findings indicate that combining CT-based representation, NearMiss-based data balancing, tuned ISUD feature selection, and Knowledge Distillation provides a computational framework for pairwise VHPI prediction.

Endothelial Matrin-3 Limits Cellular Senescence and Lipid Dysregulation to Protect Against Murine Atherosclerosis.

It is found that Matrin-3 silencing induces endothelial senescence, characterized by reduced cell proliferation, increased senescence-associated β-galactosidase activity, and impaired mitochondrial function in vitro, and that endothelial Matrin-3 deficiency is associated with both local vascular alterations and systemi...

Towards the construction of a chitin-binding domain BioBrick®: PCR Amplification of the Pseudomonas aeruginosa chiC chitin-binding domain

Historically, the chitin-binding domain (CBD) of the PA01 strain of Pseudomonas aeruginosa, Chitinase C (ChiC), has been employed as a protein tag in purification processes. However, there remains a need for CBD tags with new properties, such as increased temperature stability or binding affinity. The CBD of PAO1 ChiC...

Functional genetics reveals a role for the proteasome in regulating centromere assembly

Centromeres link chromosomes to spindle microtubules to direct their segregation during mitosis. The vertebrate centromere is built on two critical DNA-binding proteins, the histone H3-variant CENP-A and histone fold protein CENP-T. CENP-T is a potent scaffold for kinetochore assembly, such that concentrating it at ect...

An m5C-associated NSUN5–YBX1–CENPF programme marks melanoma cell states and immune features

Background RNA 5-methylcytosine (m 5 C)-associated programmes may help characterise melanoma cell states and their immune context. We investigated an NSUN5–YBX1–CENPF (NYC) programme using patient-matched sequencing, experimental perturbation and multiscale transcriptomic analyses. Methods We analysed m 5 C-MeRIP-seq a...

Principles, methods, and computational approaches to enhance protein stability and solubility

Methyltransferase RsmB chaperones E. coli ribosome assembly by orchestrating the recruitment of ribosomal proteins

Ribosomal RNA modification enzymes increase cellular fitness, but it’s little known whether this benefit arises from rRNA modification or a chaperone function of the modification enzyme. Here, we show that E. coli methyltransferase RsmB chaperones the initial recruitment of ribosomal proteins to the 16S 3′ domain rRNA,...

E8 Root Vector Encoding of Genetic Codon Degeneracy and Stereoalignment — E8 Intelligence Research

The 240 E8 root vectors encode the 64-codon genetic code's degeneracy pattern through a φ-recursive hierarchical mapping: the 20 amino acid assignments (plus stop) follow the same norm-squared distribution (1:2:4:8:16:32...) as the prime-indexed Fibonacci harmonics, with degeneracy levels 1→2→4→6→4→2 corresponding to t...