Whole-genome sequencing (WGS) permits high-resolution comparison of Mycobacterium avium subsp. paratuberculosis (MAP) isolates and pangenome analysis. Combined with animal-movement data, WGS can support transmission inference, but resolution alone does not establish the biological meaning of genomic variation. This foc...
Cleverson de Souza· Comparative Immunology, Micr...· 0 citations
Background Mitotic-figure detectors are usually evaluated by detection accuracy, but use also depends on confidence and count reliability under domain shift. Methods We evaluated MIDOG++ with leave-one-domain-out testing across seven domains. Adaptive-bin detection expected calibration error (D-ECE1) measured the bin-m...
Cleverson de Souza· Journal of Pathology Informa...· 0 citations
The reviewed evidence supports disease-history- and stage-associated differences in macrophage responsiveness, dissociation between inflammatory signaling and viable MAP control in defined models, altered lipid and phagosomal/endosomal readouts, and bacterial determinants of intracellular fitness.
Cleverson de Souza· Veterinary Immunology and Im...· 0 citations
Objective
To characterize the timing and between-cow consistency of iron-handling transcript changes after lipopolysaccharide (LPS) exposure in primary bovine monocyte-derived macrophages.
Methods
Macrophages from 5 adult Holstein donor cows were exposed to Escherichia coli LPS or PBS and profiled by RNA sequencing a...
Kevin Hall, Leah Dunston, Donghee Lee et al.· American Journal of Veterina...· 0 citations
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