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D. M. Melo

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#gene editing Open access Sep 2026

In vitro validation of a CRISPR/Cas9 guide RNA targeting COL1A1 gene by cleavage assay.

Functional validation of the gRNAs designed to target exon 2 of the COL1A1 gene is confirmed through in vitro cleavage activity and support its candidate selection for subsequent cellular studies targeting COL1A1, representing an initial step toward future knockout-style cellular models of Osteogenesis Imperfecta.

D. M. Melo, Sabrina Rodrigues Sarquis, Paolla Manzoli Luz et al. · 0 citations

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