Analysis of SpCas9 on- and off-target effects in high efficiency multiplex editing in Arabidopsis
RNA-guided nucleases (RGNs), such as Cas9 from Streptococcus pyogenes (SpCas9), are widely used for plant genome editing. Previous surveys for off-targeting, the modification of unintended targets with similarity to the intended target, indicate high specificity of SpCas9 in plant cells. However, off-targeting has not...
J. Stuttmann, J. Keilwagen, Philipe Ortet et al.
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