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ABSTRACT Enzyme turnover number (kcat) is a central kinetic parameter for biocatalysis, but experimental determination is low‐throughput and existing computational methods inadequately model enzyme–reaction interplay. Here, we introduced MCKcat, a deep learning framework that integrates multi‐scale convolutional featur...
Results indicated that AuHex92-pNP-GlcNAc exhibited a stable, compact conformation, which enhanced its substrate affinity and transglycosylation activity, which establishes a foundation for producing (GlcNAc)3 in the food, chemical, and pharmaceutical industries.
The engineered strain achieved a marked increase in 1,4-butanediamine production, demonstrating that promoter editing via ABE can effectively regulate metabolic flux and enhance the production of the target product, as well as serving as a valuable reference for the biosynthesis of other high-value-added chemicals.
Lignocellulose is an abundant renewable feedstock for biofuels and value-added bioproducts, yet its efficient bioconversion is hindered by the recalcitrant lignin barrier. While laccases show great potential for lignin modification and delignification, their limited thermostability restricts their application in high-t...
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