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Recovery of Neohesperidin and Other Bioactive Flavonoids from Bitter Orange By-Products: Impact of Hydrogen Bond Donors Using NADES-UAE as a Sustainable Approach
Natural deep eutectic solvents (NADES) have been increasingly explored as alternative extraction media for recovering high-value phytochemicals from agro-industrial byproducts. In this study, a 5 × 22 factorial design was used to evaluate the influence of HBD type, molar ratio, and added water content for choline chlor...
Cloning and Protein Expression of CpEst in Komagataella phaffii.
This chapter presents a methodology for the heterologous expression of Carica papaya esterase in the yeast Komagataella phaffii, highlighting the advantages of using a yeast-based system for scalable production and extracellular secretion.