TL;DR
This chapter presents a methodology for the heterologous expression of Carica papaya esterase in the yeast Komagataella phaffii, highlighting the advantages of using a yeast-based system for scalable production and extracellular secretion.
Lignocellulosic biomass is a promising source of sugars, however, during hydrolysis the toxic compounds are released, that can inhibit the producing strain. For today there is no data on the genetic regulation in C. famata of tolerance to inhibitors (furfural, hydroxymethylfurfural (HMF), and acetic acid), as well as w...
Mureidomycin A is a uridyl peptide antibiotic with potent bioactivity against the Gram-negative pathogen Pseudomonas aeruginosa. Crucial bottlenecks impeding further drug development include low compound production in native producers and heterologous hosts, as well as the formation of numerous derivatives, which fur...
It was seen that free pectinase is stable up to 70°C with the half-life of 7.4 min which emphasizes its thermal stability at higher temperature, which has significant impact on industrial applications.
The high-resolution structure of the T. aurantiacus xylanase TaXyn10 expressed in Escherichia coli shows that recombinant production combined with split-intein-based purification recovers the native fold of TaXyn10 and permits the formation of an N-terminal pyroglutamate. These results demonstrate that this approach ca...
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