Giovanni Maglia

Expression and Purification of CytK Nanopores.

Purification and Oligomerization of YaxAB Nanopores.

Engineered MspA Nanopores for Real-Time Detection of Insulin, Its Pharmaceutical Analogues, and Degradation Products

Nanopore sensors have demonstrated single-molecule resolution for nucleic acids and small molecules, yet the discrimination and detection of folded proteins in complex biological matrices remains a challenge. Insulin is a demanding test case: it circulates in blood at picomolar concentrations, differs from clinically...

Tunable DNA-Stabilized Bicelles as Nanoscale Membrane Mimetic Systems.

Membrane proteins are central to cellular function and constitute the majority of drug targets, yet their structural and functional characterization at the single-molecule level requires stabilization within a native-like lipid environment. Here, we introduce a robust and tunable DNA origami nanodisc that incorporates...