Coupling a developmental promoter to CRISPR interference for Wnt pathway regulation in human pluripotent stem cells
A T/Brachyury promoter to CRISPR interference targeting CTNNB1, which encodes the canonical Wnt signaling mediator β-catenin, provides a proof of concept for combining a developmental promoter with a programmable intracellular regulator and identifies a strategy for separating circuit integration from unintended target...