Systematic Development of a Compact Genome-Editing Tool Leveraging the TAM-Independent DNA Nuclease TasR in Bacillus subtilis
This study constructed a novel miniature genome editing system in B. subtilis using the TAM-independent nuclease TasR as the core component and developed the pBsu-SRP (Scissors-Rock-Paper) iterative editing system, which automatically cures the editing plasmid from the previous round while performing a new round of gen...