This study constructed a novel miniature genome editing system in B. subtilis using the TAM-independent nuclease TasR as the core component and developed the pBsu-SRP (Scissors-Rock-Paper) iterative editing system, which automatically cures the editing plasmid from the previous round while performing a new round of gene editing.
A one-step CRISPR-based transformation protocol developed in this study enables fast genome editing workflows with minimal hands-on time and demonstrates the versatility of the modified system for this industrially important genus.
Maximilian Hilkmann, Norma Welsch, M. F. Felle et al.· Applied Microbiology and Bio...· 0 citations
ABSTRACT Agrobacterium tumefaciens is a powerful delivery tool for plant genetic transformation and a pivotal platform for agricultural biotechnology. However, laboratory strains of Agrobacterium have remained genetically unoptimized for decades, with genome engineering hindered by inefficient, endogenous homologous re...
Species of Cronobacter are emerging foodborne pathogens that pose a significant threat to neonates. Functional genomic studies in Cronobacter have been hindered by the lack of efficient genetic manipulation tools. Here, we established a CRISPR/Cas9-based genome editing platform for Cronobacter. We developed a dual-plas...
Shu-Wei Yan, Jing Zhang, Yu-Fei Han et al.· Pathogens· 0 citations
An enhanced CgCas12n system (eCgCas12n) is established that enables efficient mammalian genome editing and provides an efficient genome- and base-editing platform for functional genetic studies.
Bo-Wen Hou, Xin-Yi Liu, Jia-Wen Fan et al.· Molecular Systems Biology· 0 citations
Recent discoveries of non-CRISPR RNA-guided nucleases have expanded the landscape of programmable genome editing beyond canonical Cas systems. Among these, TnpB proteins derived from IS200/IS605-family transposable elements are ultra-compact RNA-guided DNA endonucleases with potential for delivery-constrained genome en...
Bin Ma, Yi-Miao Li, Dong-Sheng Zhang et al.· Frontiers in Genome Editing· 0 citations
Genome editing in avian systems has largely relied on plasmid-based CRISPR systems and antibiotic selection to achieve successful editing. However, plasmid delivery can result in prolonged nuclease expression and potential unintended DNA integration. In addition, selection-based enrichment may mask the intrinsic geno...
Sydney G. Bingham, Jin Lee Kim, Kiho Lee et al.· Journal of Animal Science· 0 citations
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