Centromeres link chromosomes to spindle microtubules to direct their segregation during mitosis. The vertebrate centromere is built on two critical DNA-binding proteins, the histone H3-variant CENP-A and histone fold protein CENP-T. CENP-T is a potent scaffold for kinetochore assembly, such that concentrating it at ect...
Océane Marescal, Kuan-Chung Su, Matteo Di Bernardo et al.· bioRxiv· 0 citations
This work defines the requirements for cell-substrate adhesion in cultured human cells using orthogonal, large-scale functional genetic approaches and identifies established and uncharacterized regulators of adhesion, including genes involved in mitosis, focal adhesions, actin regulation, and membrane trafficking.
Kaitlyn Manzer, Kuan-Chung Su, Matteo Di Bernardo et al.· bioRxiv· 0 citations
The regulation of protein stability is essential for cellular homeostasis and is determined by a combination of intrinsic sequence motifs and extrinsic recognition enzymes. Despite growing knowledge of the protein degradation machinery, the ability to predict a protein’s stability from its amino acid sequence remains c...
Océane Marescal, Iain M. Cheeseman· bioRxiv· 0 citations
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