Nucleus-forming jumbo phages import a phage-encoded non-virion RNA polymerase (nvRNAP) into a proteinaceous nucleus, but how it is selected for import is unknown. Here, we identify an import pathway that couples nvRNAP subunit interactions with phage-encoded import factors. Maximal nvRNAP import requires a novel factor...
Claire Y. Kokontis, Deepto Mozumdar, Daphne F. Chen et al.· bioRxiv· 0 citations
A broad-acting mechanism that allows Phikmvvirus phages to evade nuclease targeting is described and phage escape through faithful repair is described and a potentially interesting gene for phage therapy is identified.
Together, phage TnSeq is a scalable technology that can identify essential phage genes, generate knockouts in all non-essential genes, and sensitively assign the quantitative fitness contributions of every gene in parallel.
A. Chan, Wearn-Xin Yee, Deepto Mozumdar et al.· Cell· 2 citations
This work investigated how the ϕKZ-encoded non-virion RNA polymerase (nvRNAP) is selectively imported and discovered that Imp7 does not directly bind to nvRNAP in vitro, suggesting multiple potential models for nvRNAP import.
Iris Zheng, J. Bondy-Denomy, Daphne F. Chen et al.· Journal of Immunology· 0 citations
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