Intrinsically disordered regions are widespread in RNA-processing machines. In Escherichia coli, RNase E uses its intrinsically disordered C-terminal domain (CTD) to recruit RNAs to the N-terminal catalytic domain, including mRNAs targeted by regulatory small RNAs (sRNAs), but the basis of substrate recognition and spe...
Daniel G. Mediati, Saleh Alquethamy, Christopher Jin et al.· bioRxiv· 2 citations
CRISPR undoubtedly transformed genome editing, but it left two crucial problems unsolved. Cas nucleases cut the chromosome and leave the cell to repair the break, an error-prone process that is inefficient for installing new DNA, and Cas9 is large relative to the cargo that viral vectors can carry for gene therapy. A n...
Christopher Jin, J. Tree· Microbiology Australia· 1 citation
Findings indicate that the regulatory fate of an sRNA–mRNA interaction is an emergent property of its biophysical context and protein-binding environment, rather than a direct consequence of physical pairing alone.
F. Safari, Daniel G. Mediati, Saleh Alquethamy et al.· bioRxiv· 0 citations
Horizontal gene transfer introduces foreign DNA that can disrupt cellular processes and is therefore subject to xenogeneic silencing by nucleoid-associated proteins such as H-NS and Hha. In Enterohaemorrhagic Escherichia coli (EHEC), prophages make up a large fraction of the accessory genome and encode many virulence f...
Pranita Poudyal, Brandon M. Sy, Daniel G. Mediati et al.· PLoS Pathogens· 0 citations
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