Koh Takeuchi

Molecular mechanism of SIRT1 activation by polymethoxyflavonoids derived from citrus peels.

Sirtuin 1 (SIRT1) activation by small molecules is linked to aging-related health benefits. We investigated how citrus peel-derived polymethoxyflavonoids (PMFs), 3,5,6,7,8,3',4'-heptamethoxyflavone (HMF) and 5,6,7,8,3',4'-hexamethoxyflavone (nobiletin), activate SIRT1. Both compounds modestly but reproducibly increased...

Expression and Purification of a Multidrug Transporter PfMATE for Structural Analyses Using Solution NMR.

A Repurposed S-Adenosyl-l-Methionine Synthetase Mediates Cytidyl-Cyclitol Natural Product Assembly.

Nucleoside natural products exhibit diverse chemical architectures and potent biological activities, yet the biosynthetic strategies that generate their structural diversity remain incompletely understood. Here, we elucidate the early stage biosynthetic pathway of the cytidyl-cyclitol natural product K-563 and its deri...