Leonel Malacrida

Linear Combination Properties of Spectral Phasors Allow Thermodynamic Analysis of Reversible Denaturation of Multidomain and Membrane Proteins

Denaturation of small globular proteins is often described as a reversible two-state transition between native and denatured conformations. Conversely, multidomain and membrane proteins frequently populate several partially folded intermediate states during denaturation. Traditional thermodynamic analysis of fluorescen...

Unveiling biomolecular condensate physiology and pathology using spectral and time-resolved fluorescence microscopy of DAN probes

Biomolecular condensates are central to cellular physiology and disease. Understanding their molecular organization and regulation requires methods that go beyond intensity-based fluorescence microscopy to capture the environmental variables that define phase behavior. In this review, we highlight the use of environmen...

Biomolecular Condensates, Crowding, and Membranes: The Role of Water Revealed with Advanced Fluorescence Microscopy and Phasor Analysis.