A generalizable method for the purification and biophysical characterization of bacterial membrane receptors
Abstract Membrane‐embedded bacterial receptors are challenging to express and purify in soluble form, yet their isolated domains are essential tools for structural and ligand‐discovery studies. Pseudomonas aeruginosa relies on the TonB‐dependent heme receptor HasR for iron acquisition, a process central to its pathogen...
Federico Bosetto, M. Zacharopoulou, M. Bycroft et al.
· Protein Science · 0 citations