#gene editing
Aug 2026
CRISPR/Cas9 Editing of Human Keratinocytes for Organotypic Modeling of Genetic Epidermal Disorders.
Step-by-step methods for deploying CRISPR/Cas9 to engineer a TERT-immortalized human epidermal keratinocyte line (N/TERT-2G) to generate monoclonal heterozygous and homozygous knockout cell lines are described.
Anthony Coon, Jessica L. Ayers, Christopher Cole et al.
· Methods in molecular biology · 0 citations