Author

Madigan M. Reid

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Review Sep 2026

VINE-seq and MultiVINE-seq for single-nucleus and multiome profiling of the brain vasculature.

VINE-seq provides a robust, reproducible workflow for the enrichment and high-resolution profiling of vascular, perivascular, and immune cells from fresh or frozen human and mouse brain tissue and optimized extraction of nuclei from purified vessels using enzymatic digestion.

Jane Oberhauser, Bella Ding, Madigan M. Reid et al. · Nature Protocols · 0 citations