Maria Zacharopoulou

A generalizable method for the purification and biophysical characterization of bacterial membrane receptors

Abstract Membrane‐embedded bacterial receptors are challenging to express and purify in soluble form, yet their isolated domains are essential tools for structural and ligand‐discovery studies. Pseudomonas aeruginosa relies on the TonB‐dependent heme receptor HasR for iron acquisition, a process central to its pathogen...

Programmable DNA-peptide nanostructures for multivalent regulation of intracellular signalling

Silane-Enriched Conducting Polymer Thin Films Improve Supported Lipid Bilayer Formation.

Supported lipid bilayers (SLBs) on conductive polymer devices provide a powerful platform for studying membrane processes, enabling cost-effective, label-free, high-throughput bioelectronic sensing. However, their reproducible formation, particularly from native membrane components, remains challenging. Here, we invest...

A generalisable method for the purification and biophysical characterisation of bacterial membrane receptors

A generalizable workflow for producing soluble receptor domains from membrane proteins is established and validated HasR constructs suitable for downstream ligand-screening applications, including aptamer and nanobody discovery.