Influence of the lipid thioether modification and formulation method on lipoplex structure, stability and transfection efficiency in HEK293T cells.
Lipoplexes, formed by electrostatic interactions between nucleic acids and cationic lipids, must protect their cargo, enable cellular uptake and promote intracellular release for non-viral gene delivery. Although traditionally produced by bulk complexation, scalable microfluidic methods are now available. However, how...