Towards the construction of a chitin-binding domain BioBrick®: PCR Amplification of the Pseudomonas aeruginosa chiC chitin-binding domain
Historically, the chitin-binding domain (CBD) of the PA01 strain of Pseudomonas aeruginosa, Chitinase C (ChiC), has been employed as a protein tag in purification processes. However, there remains a need for CBD tags with new properties, such as increased temperature stability or binding affinity. The CBD of PAO1 ChiC...