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#protein folding Open access Sep 2026

Transcriptional reprogramming with endogenous cis- and trans-elements for efficient food protein expression from safe substrates in Komagataella phaffii.

Sustainable economy and social development require a paradigm shift in the modes of production of edible proteins. Komagataella phaffii is a preferred host for food protein expression. However, its methanol-dependent production mode and host improvement with exogenous bioelements pose challenges for the manufacturing a...

Yun-Hao Li, Jia-Ying Yang, Liu-Fei Tao et al. · 0 citations
Open access Aug 2026

Evolutionary Adaptation and Targeted Metabolic Engineering Synergistically Improve Methanol‐Derived Single‐Cell Protein Production in Pichia pastoris

Methanol is a promising renewable C1 feedstock for sustainable single‐cell protein (SCP) production. However, its inherent cytotoxicity and metabolic trade‐offs between cell growth and protein synthesis remain significant bottlenecks. Here, we established an “evolutionary‐rational” dual‐driven paradigm to construct a h...

Chong Xie, Cheng-Chao Zhu, Jun-Ze Liu et al. · 0 citations

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