101. Assessment of Published Bovine Guide RNA Design Practices.
CRISPR/Cas9 is the most widely applied gene-editing (GnEd) technology in livestock, with more than 200 published studies reporting the generation of live, gene-edited animals for agricultural applications. This system consists of a Cas9 nuclease that cleaves DNA and a 19-20 base pair guide RNA (gRNA) that directs the...