Same hydrolase knockouts, different outcomes: CRISPR/Cas9 editing workflow shapes resulting cellular phenotype and transcriptome in CHO
CRISPR/Cas9-based multi-gene knockout strategies are widely applied to engineer Chinese hamster ovary (CHO) cells, yet the impact of editing workflow on cellular outcomes remains poorly understood. Here, we systematically compare sequential and multiplex genome editing approaches to generate CHO cell lines lacking nine...
Linus Weiss, Stefan Schneider, Kerstin Otte et al.
· Frontiers in Bioengineering... · 0 citations