Open access
Jul 2026
Structure and evolution-guided design of minimal RNA-guided nucleases.
A protein design strategy is used that couples a structure-guided inverse-folding model with evolution-informed residue constraints to generate active, divergent variants of TnpB, a minimal CRISPR-Cas12-like nuclease, termed SynTnpBs, establishing a strategy for creating non-natural RNA-guided nucleases and conformationally active nucleic acid binders, enlarging the designable protein space.
Petr Skopintsev, Isabel Esain-Garcia, Evan C. DeTurk et al.
· Science · 2 citations