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Author

T. Kashima

2 papers indexed here

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Open access Sep 2026

GH172 Difructose Dianhydride I Synthase: A Two-Stepped Journey via Glycosylation and Cyclization Using QM/MM Metadynamics.

The difructose dianhydride I synthase/hydrolase α FFase 1 catalyzes the reversible transformation of inulobiose into difructose dianhydride I, a component of caramel. The proposed reaction mechanism of such a transformation consists of a glycosylation step followed by a cyclization to yield the dianhydride from the dis...

Santiago Alonso-Gil, Bruno Di Geronimo, T. Kashima et al. · 0 citations
Open access Sep 2026

Structural mechanism defining product specificity in glycoside hydrolase family 66 cycloisomaltotetraose glucanotransferase

The crystal structures of CI4Tase are determined and the strict size-control mechanism of CI4Tase is elucidated, providing a structural foundation for engineering cycloisomaltooligosaccharide-producing enzymes with optimized transglycosylation efficiency and specificity for industrial applications.

Ryota Yasukochi, T. Kashima, Tetsuya Mori et al. · 0 citations

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