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Thomas J. Begley

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Open access Sep 2026

Stress-Induced Reprogramming of tRNA Epitranscriptome in the Industrially Important Yeast Komagataella phaffii

Transfer RNA (tRNA) modifications regulate codon-biased translation and enable rapid translational adaptation to environmental stresses in diverse organisms. While these mechanisms have been extensively characterized in Saccharomyces cerevisiae, little is known about the epitranscriptomic landscape of nonconventional...

Alexander J. B. Reading, Chi-Kong Chan, R. Raman et al. · 0 citations
Open access Sep 2026

Multi-dimensional DNA nanostructures isothermally assembled in hydrated ionic liquids

This work develops a potential method to construct more biostable DNA nanostructures and 3D crystals in a simple one-tube process by assembly of a wide variety of DNA nanostructures and 3D crystals in a hydrated ionic liquid instead of magnesium at constant moderate temperatures.

Hannah Talbot, Lauren Nguyen, Dhanush Gandavadi et al. · 0 citations
#protein folding Sep 2026

Improving Yield and Assessing Quality of Secreted Proteins Over-Expressed in Pichia Pastoris with Chaperones and Structural Mass Spectrometry

It is demonstrated that maximizing recombinant protein production requires optimization of both yield and structural quality and establish complementary strategies for improving secreted protein expression in Pichia.

Xin-Zhe Ren, E-Ming Rau, Lauren Coutu et al. · 0 citations

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