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Tian Zhang

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Open access Sep 2026

A GWAS-identified U-box E3 ligase MsPUB6 is positively regulated by MsDREB1C and contributes to drought tolerance in alfalfa.

U-box-type E3 ubiquitin ligases (PUBs) are critical for signaling homeostasis and abiotic stress responses via substrate ubiquitination, yet their upstream transcriptional regulation remains largely unexplored, especially in alfalfa drought adaptation. Through GWAS of 171 alfalfa accessions, we identified a novel U-box...

Yang-Yang Zhang, Xue-Qian Jiang, Hua-Juan Leng et al. · 0 citations
#gene editing Open access Sep 2026

Converging GWAS and Genome Editing Decrypts MsCBF8‐ MsGolS2 Regulatory Module for Drought Tolerance Without Yield Penalty in Medicago sativa

ABSTRACT Drought constitutes a major abiotic stress limiting alfalfa productivity. To elucidate the genetic basis of drought tolerance, a genome‐wide association study (GWAS) was conducted in alfalfa, which identified a CBF/DREB1 family transcription factor designated MsCBF8. Utilizing an optimized CRISPR‐Cas9 system,...

Yang-Yang Zhang, Xue-Qian Jiang, Tian Zhang et al. · 0 citations

Genome-wide identi fi cation and characterization of DCL , AGO , and RDR gene families and their abiotic stress responses in alfalfa ( Medicago sativa L.)

The first genome-wide analysis of these gene families in autotetraploid alfalfa provides a theoretical foundation for future functional studies of RNA silencing pathway genes and offer valuable genetic resources for the molecular breeding of stress-resilient alfalfa.

Ming Xu, Hao Liu, Kai Zhu et al. · 0 citations

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