U-box-type E3 ubiquitin ligases (PUBs) are critical for signaling homeostasis and abiotic stress responses via substrate ubiquitination, yet their upstream transcriptional regulation remains largely unexplored, especially in alfalfa drought adaptation. Through GWAS of 171 alfalfa accessions, we identified a novel U-box...
ABSTRACT Drought constitutes a major abiotic stress limiting alfalfa productivity. To elucidate the genetic basis of drought tolerance, a genome‐wide association study (GWAS) was conducted in alfalfa, which identified a CBF/DREB1 family transcription factor designated MsCBF8. Utilizing an optimized CRISPR‐Cas9 system,...
The first genome-wide analysis of these gene families in autotetraploid alfalfa provides a theoretical foundation for future functional studies of RNA silencing pathway genes and offer valuable genetic resources for the molecular breeding of stress-resilient alfalfa.
Ming Xu, Hao Liu, Kai Zhu et al.· 0 citations
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