Combinatorial Optimization of Promoters and Anchoring Proteins Drives a 10-Fold Enhancement in Yeast Surface Display of eGFP
The pYGAL1-Sed1p-eGFP-C system not only provides a robust and highly efficient chassis for displaying eGFP but also holds significant promise as a versatile scaffold for the surface presentation of diverse heterologous proteins in yeast, thereby expanding the utility of YSD in biotechnology and synthetic biology.