Label-Free and Strain-Resolved Characterization of AI-2 Associated Quorum Sensing Functional Phenotypes in Bacterial Isolates Using Single-Cell Raman Spectroscopy and Deep Learning
A rapid, label-free SCRS-based model for characterizing AI-2-associated QS functional phenotypes in bacterial isolates is provided and integrated Gradients analysis highlighted coordinated Raman spectral regions associated with proteins, lipids, polysaccharides, nucleic acids, and sulfur-containing groups.